The influence of Lactobacillus casei DN 114 001 on the activity of faecal enzymes and genotoxicity of faecal water in the presence of heterocyclic aromatic amines.
Authors: Nowak A1, Sliżewska K2, BÅ‚asiak J3, Libudzisz Z2.
Abstract: High activity of bacterial enzymes in human colon and genotoxicity of faecal water (FW) are biomarkers of the harmful action of microbiota. The aim of the present study was to assess the activity of β-glucuronidase and β-glucosidase and the genotoxicity of FW in vitro after incubation with 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) or 2-amino-1-methyl-6-phenyl-1H-imidazo [4,5-b]pyridine (PhIP) and probiotic Lb. casei DN 114 001 (Actimel). Our results indicate, that IQ and PhIP greatly increased the activity...
Lactic acid bacteria pre-challenged by simulated gastrointestinal conditions is a suitable approach for study of potential probiotic properties.
Authors: Huang HY1, Hsieh HY1, King VA2, Chi LL3, Tsen JH4.
Abstract: Some lactic acid bacteria (LAB) possess probiotic properties beneficial to human health. However, both gastric acid and bile salt have adverse influences on the survival and viability of ingested probiotics. This research investigated the changes in potential probiotic properties of LAB strains after treatment with simulated gastrointestinal (GI) conditions. Three strains of LAB, namely Lactobacillus acidophilus BCRC 10695, Lactobacillusparacasei BCRC 14023, and Bifidobacterium bifidum BCRC 14615, were used...
Probiotic Bacillus pumilus SE5 shapes the intestinal microbiota and mucosal immunity in grouper Epinephelus coioides.
Authors: Yang HL1, Xia HQ, Ye YD, Zou WC, Sun YZ.
Abstract: The health benefits of probiotics are thought to occur, at least in part, through an improved intestinal microbial balance in fish, although the molecular mechanisms whereby probiotics modulate the intestinal microbiota by means of activation of mucosal immunity are rarely explored. In this study, the effects of viable and heat-inactivated probiotic Bacillus pumilus SE5 on the intestinal dominant microbial community and mucosal immune gene expression were evaluated. The fish were fed for 60 d with 3 different...
Authors: Vitetta L1, Bambling M, Alford H.
Abstract: Mental health is closely linked to physical health. Depression (e.g., major depression) is highly prevalent worldwide and a major cause of disability. In a subgroup with treatment-resistant depression, standard pharmacotherapy interventions provide small if any incremental improvement in patient outcomes and may also require the application of an alternate approach. Therefore, in addition to the standard pharmacotherapies prescribed, patients will also be advised on the benefits of psychological counseling,...
Lactobacillus helveticus SBT2171 Inhibits Lymphocyte Proliferation by Regulation of the JNK Signaling Pathway.
Authors: Hosoya T1, Sakai F1, Yamashita M1, Shiozaki T2, Endo T3, Ukibe K1, Uenishi H1, Kadooka Y1, Moriya T1, Nakagawa H2, Nakayama Y2, Miyazaki T2.
Abstract: Lactobacillus helveticus SBT2171 (LH2171) is a lactic acid bacterium with high protease activity and used in starter cultures in the manufacture of cheese. We recently reported that consumption of cheese manufactured using LH2171 alleviated symptoms of dextran sodium sulfate (DSS)-induced colitis in mice. In this study, we have examined whether LH2171 itself exerts an inhibitory effect on the excessive proliferation of lymphocytes. We found that LH2171 inhibited the proliferation of LPS-stimulated mouse T and...
Selection and Characterization of Cheonggukjang (Fast Fermented Soybean Paste)-Originated Bacterial Strains with a High Level of S-adenosyl-L-methionine Production and Probiotics Efficacy.
Authors: Park S1, Kim MJ, Hong J, Kim HJ, Yi SH, Lee MK.
Abstract: This study was executed to develop probiotics producing S-adenosyl-L-methionine (SAMe), a methyl group donor in the 5-methyltetrahydrofolate methylation reaction in animal cells. SAMe is an essential substance in the synthesis, activation, and metabolism of hormones, neurotransmitters, nucleic acids, phospholipids, and cell membranes of animals. SAMe is also known as a nutritional supplement for improving human brain function. In this study, SAMe-producing strains were identified in six kinds of Cheonggukjang,...
Oligosaccharides composition in eight Food Legumes species as detected by high resolution mass spectrometry.
Authors: Fan PH1, Zang MT, Xing J.
Abstract: BACKGROUND: As probiotics, soy oligosaccharides become popular as healthy foods to reduce disease risk. However, comprehensive information about oligosaccharides in different food legumes is limited.RESULTS: In this study, eight oligosaccharides were well-detected and quantified in different varieties of eight legume species using high resolution mass spectrometry. It was determined that species could be distinguished by total content of oligosaccharides and their distribution modes. Among the studied species,...
Authors: Oakley BB1, Lillehoj HS, Kogut MH, Kim WK, Maurer JJ, Pedroso A, Lee MD, Collett SR, Johnson TJ, Cox NA.
Abstract: The domestic chicken is a common model organism for human biological research, and of course also forms the basis of a global protein industry. Recent methodological advances have spurred the recognition of microbiomes as complex communities with important influences on the health and disease status of the host. In this mini-review, we provide an overview of the current state of knowledge of the chicken GI microbiome focusing on spatial and temporal variability, the presence and importance of human pathogens,...
Probiotics' Preventive Effect on Pediatric Food Allergy: A Meta-analysis of Randomized Controlled Trials.
Authors: Kong XY, Yang Y, Guan J, Wang RZ.
Abstract: Objective To investigate the preventive effect of probiotics on pediatric food allergy. Methods From MEDLINE bibliographical database, we searched and reviewed all randomized controlled trials on the preventive effects of probiotics on pediatric food allergies up to September 2013 and excluded the studies that do not meet inclusion criteria and extracted the data. Meta-analysis for the results of homogenous studies was performed using RevMan 5.0 and the co-effect was pooled by using fixed-effects model of relative...
Authors: Rodrigues F1, Maia MJ, das Neves J, Sarmento B, Amaral MH, P P Oliveira MB.
Abstract: Objective: The aim of this study was to develop and characterize suppositories for vaginal delivery of Lactobacillus acidophilus. Methods: Formulations were performed in order to select suitable excipients based on suppository formation feasibility and cytotoxicity. Solid body and hollow-type suppositories were prepared by melting and molding using poly(ethylene glycol) (PEG) 400 and 4000 or Witepsol (WIT) H12 as excipients. L. acidophilus was incorporated in the molten mass before molding solid body suppositories...
Effect of probiotic VSL#3 in the treatment of minimal hepatic encephalopathy- a non-inferiority randomized controlled trial.
Authors: Pratap Mouli V1, Benjamin J, Bhushan Singh M, Mani K, Garg SK, Saraya A, Joshi YK.
Abstract: BACKGROUND: Minimal hepatic encephalopathy (MHE) impairs daily functioning and health related quality of life in chronic liver disease (CLD). Lactulose is the standard treatment but has side effects; Probiotics have encouraging role in MHE.AIM: To test whether probiotics is non-inferior to lactulose in improving MHE.METHODS: Patients with CLD (n=227) were screened for MHE using neuropsychometrics tests - Number Connection Tests A and B (or Figure Connection Tests A and B) and/or neurophysiologic test - P-300...
Authors: Bridgman SL, Azad MB, Field CJ, Letourneau N, Johnston DW, Kaplan BJ, Kozyrskyj AL.
Abstract: BACKGROUND: Probiotic products that may modify the intestinal microbiota are becoming increasingly available and known to consumers due to their potential to prevent or treat many pediatric health conditions. As scientific knowledge of the health benefits of probiotics increases, it is important to identify factors that may prevent their successful integration into patient care as well as to ensure effective translation of research findings. The aim of this study was to describe maternal perspectives on probiotics...
Authors: Mitchel EB1, Lavine JE.
Abstract: BACKGROUND: Paediatric nonalcoholic fatty liver disease (NAFLD) is a major public health concern given the recent increase in its prevalence and link to obesity and other metabolic comorbidities. Current treatment strategies involve lifestyle changes. Other surgical and pharmacologic interventions have been proposed; however, limited randomised controlled trials (RCTs) in the paediatric population restrict their use.AIM: To review the current management of paediatric NAFLD, including lifestyle and pharmacologic...
Dietary supplementation of milk fermented with probiotic Lactobacillus fermentum enhances systemic immune response and antioxidant capacity in aging mice.
Authors: Sharma R1, Kapila R2, Kapasiya M1, Saliganti V1, Dass G1, Kapila S1.
Abstract: Although probiotics are known to enhance the host immune response, their roles in modulating immunosenescence, resisting infection, and improving redox homeostasis during aging remain unclear. Therefore, the present study was devised in aging mice to assess the antiimmunosenescence potential from the consumption of milk that is fermented with probiotic Lactobacillus fermentum MTCC 5898 (LF). We hypothesized that probiotic supplementation would boost immunity, improve antioxidant capacity, and resist severity...
Authors: TulumoÄŸlu S1, Kaya HI2, SimÅŸek O3.
Abstract: The aim of this study was to characterize the probiotic characteristics of L. fermentum strains isolated from Tulum cheese. Seven L. fermentum strains were selected among the isolated and identified lactobacillus strains due to their abundance. When the gastric condition was considered, L. fermentum LP3 and LP4 were able to tolerate pH 2.5 and 1% bile salt. All L. fermentum strains had similar enzymatic activity and antibiotic resistance pattern but the highest antagonistic effect was detected within LP3, LP4...
Authors: Nielsen B1, GÃ¼rakan GC, UnlÃ¼ G.
Abstract: Kefir is a fermented dairy beverage produced by the actions of the microflora encased in the "kefir grain" on the carbohydrates in the milk. Containing many bacterial species already known for their probiotic properties, it has long been popular in Eastern Europe for its purported health benefits, where it is routinely administered to patients in hospitals and recommended for infants and the infirm. It is beginning to gain a foothold in the USA as a healthy probiotic beverage, mostly as an artisanal beverage,...
Authors: Vuotto C, Longo F, Donelli G.
Abstract: Altered bowel flora is currently thought to play a role in a variety of disease conditions, and the use of Bifidobacterium spp. and Lactobacillus spp. as probiotics has been demonstrated to be health-promoting, even if the success of their administration depends on the applied bacterial strain(s) and the targeted disease. In the last few decades, specific probiotics have been shown to be effective in the treatment or the prevention of acute viral gastroenteritis, pediatric post-antibiotic-associated diarrhea,...
A 10-Patient Case Study on the Influence of Two Different Probiotics on Individual Intestinal Microbiota.
Authors: Uchiyama-Tanaka Y.
Abstract: Background: Probiotics increase beneficial microbiota and improve chronic conditions, such as atopic diseases, irritable bowel disease, and obesity. However, because there are so many probiotics, it is unknown which probiotics might have more of an effect on intestinal microbiota. Objective: To understand how two different types of probiotics influence human intestinal microbiota, human fecal microbiota were analyzed after ingestion of each of the probiotics. Methods: Ten outpatients were enrolled. Study A:...
Authors: Reid G1, Brigidi P, Burton JP, Contractor N, Duncan S, Fargier E, Hill C, Lebeer S, Martín R, McBain AJ, Mor G, O'Neill C, Rodríguez JM, Swann J, van Hemert S, Ansell J.
Abstract: As studies uncover the breadth of microbes associated with human life, opportunities will emerge to manipulate and augment their functions in ways that improve health and longevity. From involvement in the complexities of reproduction and fetal/infant development, to delaying the onset of disease, and indeed countering many maladies, microbes offer hope for human well-being. Evidence is emerging to suggest that microbes may play a beneficial role in body sites traditionally viewed as being sterile. Although...
Comparison of Lactobacillus reuteri and Nystatin Prophylaxis on Candida Colonization and Infection in Very Low Birth Weight Infants.
Authors: Oncel MY1, Arayici S, Sari FN, Kadioglu Simsek G, Yurttutan S, Erdeve O, Saygan S, Uras N, Oguz SS, Dilmen U.
Abstract: Objective: The aim of this study was to compare the efficacy of orally administered Lactobacillus reuteri (L. reuteri) versus nystatin in prevention of fungal colonization and invasive candidiasis in very low birth weight infants. Methods: A prospective, randomized comparative study was conducted in preterm infants with a gestational age of â‰¤32 weeks and birth weight of â‰¤1500 g. Patients were randomized into two groups, to receive L. reuteri or nystatin. Skin and stool cultures were performed once...
Immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus on holoxenic mice.
Authors: Ditu LM1, Chifiriuc MC1, Bezirtzoglou E2, Marutescu L1, Bleotu C3, Pelinescu D1, Mihaescu G1, Lazar V1.
Abstract: BACKGROUND: Competition of probiotic bacteria with other species from the intestinal microbiota involves different mechanisms that occur regardless of probiotics' viability. The objective of this paper was to assess the cytokine serum levels in holoxenic mice after oral administration of non-viable components (NVC) of Enterococcus faecium probiotic culture stimulated with heat-inactivated Escherichia coli and Bacillus cereus in comparison to NVC of unstimulated E. faecium probiotic culture.METHODS: Probiotic...
Control of cell morphology of probiotic Lactobacillus acidophilus for enhanced cell stability during industrial processing.
Authors: Senz M1, van Lengerich B2, Bader J3, Stahl U4.
Abstract: The viability of bacteria during industrial processing is an essential quality criterion for bacterial preparations, such as probiotics and starter cultures. Therefore, producing stable microbial cultures during proliferation is of great interest. A strong correlation between the culture medium and cellular morphology was observed for the lactic acid bacterium Lactobacillus acidophilus NCFM, which is commonly used in the dairy industry as a probiotic supplement and as a starter culture. The cell shapes ranged...
Authors: De Greef E1, Vandenplas Y, Hauser B, Devreker T, Veereman G.
Abstract: While the role of microflora is well established in the development of inflammatory bowel disease; data is still emerging regarding irritable bowel syndrome as the knowledge on brain---gut interaction grows. For both patient groups multiple efficacious treatments exist, but there are still unmet needs as long term disease control remains an issue. Given the importance of the microbiome on the development of the immune system and the interaction between the microbiome and the host organism, this 'route' is being...
Perinatal maternal probiotic intervention impacts immune responses and ileal mucin gene expression in a rat model of irritable bowel syndrome.
Authors: Barouei J1, Moussavi M1, Hodgson DM2.
Abstract: Alterations in immune responses and intestinal secretory state are among features commonly observed in the maternal separation (MS) rat model of Irritable Bowel Syndrome. This study examined whether perinatal maternal introduction of probiotics influences plasma immune markers and ileal mucin-2 (MUC2) gene expression in rat offspring exposed to neonatal maternal separation (MS, 3 h/day, postnatal days (PND) 2-14) and/or subsequently to acute restraint stress in adulthood (AS, 30 min/day, PND 83-85). Data analysis...
Four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® shows modest effect on triacylglycerol in young healthy adults.
Authors: Bjerg AT1, Kristensen M1, Ritz C1, Stark KD2, Holst JJ3, Leser TD4, Wellejus A4, Astrup A1.
Abstract: The microbiota has been shown to have the potential to affect appetite and blood lipids positively in animal studies. We investigated if four weeks supplementation with Lactobacillus paracasei subsp. paracasei L. casei W8® (L. casei W8) had an effect on subjective appetite sensation, ad libitum energy intake, glucagon-like peptide 1 (GLP- 1), glucose and insulin response in humans. Secondarily, we explored potential effects on blood lipids, fatty acids and stearoyl-CoA desaturase-1 (SCD1) activity in humans...