Different effects of two newly-isolated probiotic Lactobacillus plantarum 15HN and Lactococcus lactis subsp. Lactis 44Lac strains from traditional dairy products on cancer cell lines.
Authors: Haghshenas B1, Abdullah N2, Nami Y1, Radiah D3, Rosli R1, Khosroushahi AY4.
Abstract: Lactobacillus and Lactococcus strains isolated from food products can be introduced as probiotics because of their health-promoting characteristics and non-pathogenic nature. This study aims to perform the isolation, molecular identification, and probiotic characterization of Lactobacillus and Lactococcus strains from traditional Iranian dairy products. Primary probiotic assessments indicated high tolerance to low pH and high bile salt conditions, high anti-pathogenic activities, and susceptibility to high consumption...
Genotyping by Randomly Amplified Polymorphic DNA of bacteriocin producing Lactobacillus acidophilus strains from Nigeria.
Authors: Alli JA1, Iwalokun BA, Oluwadun A, Okonko IO.
Abstract: Yoghurt and starter culture producers are still searching strains of Lactobacillus acidophilus to produce healthier yogurt with longer shelf life, better texture, taste and quality. This study determined the genotyping of bacteriocin producing Lactobacillus acidophilus strains recovered from Nigerian yogurts. Yogurt samples were collected from four different states of South West regions of Nigeria. Isolates were obtained from MRS Medium and biochemically characterized. This was further confirmed by API50CH....
Use of probiotics to correct dysbiosis of normal microbiota following disease or disruptive events: a systematic review.
Authors: McFarland LV.
Abstract: OBJECTIVE: To assess the evidence for the claim probiotics can correct dysbiosis of the normal microbiota resulting from disease or disruptive events.SETTING: Systematic review of published clinical trials of patients receiving a probiotic intervention for the prevention or treatment of various diseases.DATA SOURCES: Sources searched (1985-2013): PubMed, EMBASE, Cochrane Database of Systematic Reviews, CINAHL, AMED and ISI Web of Science. Three on-line clinical trial registries were searched: Cochrane Central...
Antihypertensive activity of blueberries fermented by Lactobacillus plantarum DSM 15313 and effects on the gut microbiota in healthy rats.
Authors: Ahrén IL1, Xu J2, Onning G1, Olsson C3, Ahrné S4, Molin G4.
Abstract: BACKGROUND & AIMS: The aim of the present animal study was to examine the anti-hypertensive capacity of two probiotic products combining blueberries and the tannase producing probiotic bacteria Lactobacillus plantarum DSM 15313 and to investigate if such an effect is linked to a change in the gut microbiota.METHODS: Male Sprague Dawley rats were randomly divided into six groups of nine each. Three groups of the animals were treated with NG-nitro-l-arginine methyl ester (L-NAME) in the drinking water (40Â mg/L)...
Dietary supplementation of probiotics affects growth, immune response and disease resistance of Cyprinus carpio fry.
Authors: Gupta A1, Gupta P2, Dhawan A3.
Abstract: The effects of dietary Bacillus coagulans (MTCC 9872), Bacillus licheniformis (MTCC 6824) and Paenibacillus polymyxa (MTCC 122) supplementation on growth performance, non-specific immunity and protection against Aeromonas hydrophila infection were evaluated in common carp, Cyprinus carpio fry. Laboratory maintained B. coagulans, B. licheniformis and P. polymyxa were used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. Healthy fish fry were challenged by this bacterium...
Authors: Turroni F1, Duranti S2, Bottacini F1, Guglielmetti S3, Van Sinderen D1, Ventura M2.
Abstract: Bifidobacteria are considered dominant and for this reason key members of the human gut microbiota, particularly during the first one to two years following birth. A substantial proportion of the bifidobacterial population in the intestine of infants belong to the Bifidobacterium bifidum taxon, whose members have been shown to display remarkable physiological and genetic features involving adhesion to epithelia, as well as utilization of host-derived glycans. Here, we reviewed the current knowledge on the genetic...
Interactions between amphibians' symbiotic bacteria cause the production of emergent anti-fungal metabolites.
Authors: Loudon AH1, Holland JA1, Umile TP2, Burzynski EA2, Minbiole KP2, Harris RN1.
Abstract: Amphibians possess beneficial skin bacteria that protect against the disease chytridiomycosis by producing secondary metabolites that inhibit the pathogen Batrachochytrium dendrobatidis (Bd). Metabolite production may be a mechanism of competition between bacterial species that results in host protection as a by-product. We expect that some co-cultures of bacterial species or strains will result in greater Bd inhibition than mono-cultures. To test this, we cultured four bacterial isolates (Bacillus sp., Janthinobacterium...
Authors: Morgan AR1, Han DY, Wickens K, Barthow C, Mitchell EA, Stanley TV, Dekker J, Crane J, Ferguson LR.
Abstract: BACKGROUND: In a double-blind, randomized, placebo-controlled birth cohort, we have recently shown a beneficial effect of Lactobacillus rhamnosus HN001 (HN001) for the prevention of eczema in children through to 6 years of age but no effect of Bifidobacterium animalis subsp lactis HN019 (HN019).OBJECTIVE: Among this cohort of children, we aim to investigate whether these probiotics could modify the expression of genetic predisposition to eczema conferred by genetic variation in susceptibility genes.METHODS:...
Enteral Ecoimmunonutrition Reduced Enteral Permeability and Serum Ghrelin Activity in Severe Cerebral Stroke Patients with Lung Infection.
Authors: Xu XD1, Shao F.
Abstract: The study analyzed how enteral ecoimmunonutrition, which comprises probiotics, glutamine, fish oil, and Enteral Nutritional Suspension (TPF), can impact on the enteral permeability and serum Ghrelin activity in severe cerebral stroke patients with lung infection. Among 190 severe cerebral stroke patients with tolerance to TPF, they were randomized into control and treatment groups after antibiotics treatment due to lung infections. There were 92 patients in the control group and 98 patients in treatment group....
Lactobacillus gasseri SBT2055 Induces TGF-β Expression in Dendritic Cells and Activates TLR2 Signal to Produce IgA in the Small Intestine.
Authors: Sakai F1, Hosoya T1, Ono-Ohmachi A1, Ukibe K1, Ogawa A1, Moriya T1, Kadooka Y1, Shiozaki T2, Nakagawa H2, Nakayama Y2, Miyazaki T2.
Abstract: Probiotic bacteria provide benefits in enhancing host immune responses and protecting against infection. Induction of IgA production by oral administration of probiotic bacteria in the intestine has been considered to be one reason for this beneficial effect, but the mechanisms of the effect are poorly understood. Lactobacillus gasseri SBT2055 (LG2055) is a probiotic bacterium with properties such as bile tolerance, ability to improve the intestinal environment, and it has preventive effects related to abdominal...
Authors: Miller LE.
Abstract: ABSTRACT Probiotics are live micro-organisms that confer a health benefit on the host when administered in adequate dosages and have been extensively studied for treatment of gastrointestinal (GI) disorders. While a growing body of evidence from randomized controlled trials supports the use of probiotics for a variety of GI conditions, the media and internet are saturated with advertisements falsely claiming that specific probiotic strains or strain combinations can improve, or even cure, many GI complaints....
Surface proteins of Propionibacterium freudenreichii are involved in its anti-inflammatory properties.
Authors: Le Maréchal C1, Peton V1, Plé C2, Vroland C1, Jardin J1, Briard-Bion V1, Durant G1, Chuat V3, Loux V4, Foligné B2, Deutsch SM1, Falentin H1, Jan G5.
Abstract: Propionibacterium freudenreichii is a beneficial bacterium used in the food industry as a vitamin producer, as a bio-preservative, as a cheese ripening starter and as a probiotic. It is known to adhere to intestinal epithelial cells and mucus and to modulate important functions of the gut mucosa, including cell proliferation and immune response. Adhesion of probiotics and cross-talk with the host rely on the presence of key surface proteins, still poorly identified. Identification of the determinants of adhesion...
Authors: Ishikawa KH1, Mayer MP, Miyazima TY, Matsubara VH, Silva EG, Paula CR, Campos TT, Nakamae AE.
Abstract: PURPOSE: The prevalence of Candida infections has been rising with an increasingly aging population and a larger population of immunocompromised individuals. The use of probiotics may be an alternative approach to antifungal agents in the prevention and treatment of oral candidiasis. This study aimed to evaluate the short-term effect of probiotics in reducing the infection level of oral Candida in candidiasis-asymptomatic elderly denture wearers.MATERIALS AND METHODS: In a double-blind randomized study, 59 denture...
Authors: Gholami Z, Khosravi-Darani K1.
Abstract: Conjugated linoleic acid (CLA) has attracted considerable attention in health due to its important physiological properties proved in several in vivo experiments. Many bacteria, especially some probiotics, are able to produce CLA from the linoleic acid (LA) present in milk. In this review, CLA production by microorganisms is described. Then factors on the influencing the microbial production and the initial CLA content in milk fat are introduced. After a glimpse on the content of CLA in dairy products and human...
The treatment with the probiotic Shewanella putrefaciens Pdp11 of specimens of Solea senegalensis exposed to high stocking densities to enhance their resistance to disease.
Authors: Tapia-Paniagua ST1, Vidal S2, Lobo C3, Prieto-Ãlamo MJ4, Jurado J4, Cordero H5, Cerezuela R5, García de la Banda I3, Esteban MA5, Balebona MC2, MoriÃ±igo MA2.
Abstract: Aquaculture industry exposes fish to acute stress events, such as high stocking density, and a link between stress and higher susceptibility to diseases has been concluded. Several studies have demonstrated increased stress tolerance of fish treated with probiotics, but the mechanisms involved have not been elucidated. Shewanella putrefaciens Pdp11 is a strain isolated from healthy gilthead seabream (Sparus aurata L.) and it is considered as probiotics. The aim of this study was to evaluate the effect of the...
Correction: Probiotics (VSL#3) Prevent Endothelial Dysfunction in Rats with Portal Hypertension: Role of the Angiotensin System.
Authors: The PLOS ONE Staff.
Abstract: [This corrects the article DOI: 10.1371/journal.pone.0097458.]....
Authors: Aboubakr HA1, El-Banna AA, Youssef MM, Al-Sohaimy SA, Goyal SM.
Abstract: Foodborne viruses, particularly human norovirus (NV) and hepatitis virus type A, are a cause of concern for public health making it necessary to explore novel and effective techniques for prevention of foodborne viral contamination, especially in minimally processed and ready-to-eat foods. This study aimed to determine the antiviral activity of a probiotic lactic acid bacterium (LAB) against feline calicivirus (FCV), a surrogate of human NV. Bacterial growth medium filtrate (BGMF) of Lactococcus lactis subsp....
Bacillus sp. LT3 improves the survival of gnotobiotic brine shrimp (Artemia franciscana) larvae challenged with Vibrio campbellii by enhancing the innate immune response and by decreasing the activity of shrimp-associated vibrios.
Authors: Niu Y1, Defoirdt T2, Baruah K3, Van de Wiele T4, Dong S5, Bossier P6.
Abstract: Bacteria belonging to the genus Bacillus are amongst the most intensively studied group of bacteria for use as probiotics in aquaculture. However, the exact mechanism of action of these bacteria is often not well described, and the microbiota that are naturally present in cultures of test organisms often compromise the interpretation of the results. The present study aimed to evaluate the putative probiotic effect of Bacillus sp. LT3 in a model system with gnotobiotic brine shrimp Artemia franciscana larvae....
A Systems Biology Approach Investigating the Effect of Probiotics on the Vaginal Microbiome and Host Responses in a Double Blind, Placebo-Controlled Clinical Trial of Post-Menopausal Women.
Authors: Bisanz JE1, Seney S2, McMillan A1, Vongsa R3, Koenig D3, Wong L3, Dvoracek B3, Gloor GB4, Sumarah M5, Ford B6, Herman D6, Burton JP7, Reid G8.
Abstract: A lactobacilli dominated microbiota in most pre and post-menopausal women is an indicator of vaginal health. The objective of this double blinded, placebo-controlled crossover study was to evaluate in 14 post-menopausal women with an intermediate Nugent score, the effect of 3 days of vaginal administration of probiotic L. rhamnosus GR-1 and L. reuteri RC-14 (2.5Ã—109 CFU each) on the microbiota and host response. The probiotic treatment did not result in an improved Nugent score when compared to when placebo....
Authors: Sams ER1, Whiteley M2, Turner KH3.
Abstract: The field of medical microbiology was born in the late 19th century. Robert Koch, Joseph Lister, Louis Pasteur, and others were responsible for the classic work that forms the foundation of much of today's science. This work is often taught to students today as perfectly formed ideas and experiments, without the relevant historical context. Yet science was as much a competitive and collaborative enterprise during the time of Pasteur as it is today. Therefore, to fully appreciate the contributions made by the...
Optimizing clarithromycin-containing therapy for Helicobacter pylori in the era of antibiotic resistance.
Authors: Molina-Infante J, Gisbert JP.
Abstract: The efficacy of triple therapy for Helicobacter pylori infection has dramatically declined over the last decade, largely related to increasing clarithromycin resistance rates. From a microbiological standpoint, bismuth quadruple therapy is the ideal replacement since it combines drugs for which resistance does not impair its efficacy. Nonetheless, several obstacles such as availability, complexity or tolerance prevent a general implementation of bismuth quadruple therapy, so non-bismuth quadruple regimens remain...
Pharmacological and dietary agents for colorectal cancer chemoprevention: Effects on polyamine metabolism (Review).
Authors: Linsalata M, Orlando A, Russo F.
Abstract: Chemoprevention is the long-term use of different chemical agents, both synthetic and natural, to prevent or delay the onset of disease. Since colorectal cancer has a significant environmental component, it is an ideal disease in which to evaluate the potential benefits of chemopreventive agents. The polyamines, spermine, spermidine and putrescine have been involved in almost all the steps of colorectal tumorigenesis. Consequently, polyamine biosynthesis and catabolism can be considered as promising targets...
Authors: Ruiz L1, Hevia A, Bernardo D, Margolles A, Sánchez B.
Abstract: Interest in probiotic bacteria, in the context of health and disease, is increasing and gathering scientific evidence, as is reflected by their growing utilization in food and pharma industry. As a consequence, a lot of research effort over the past few years has been dedicated to discern the molecular mechanisms responsible for their purported attributes. Remarkably, whereas the traditional probiotic concept assumes that bacteria must be alive during their administration in order to exert health promoting effects,...
Authors: Jeronymo-Ceneviva AB1, de Paula AT, Silva LF, Todorov SD, Franco BD, Penna AL.
Abstract: This study evaluated the probiotic properties (stability at different pH values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of β-galactosidase production, evaluation of the presence of genes encoding MapA and Mub adhesion proteins and EF-Tu elongation factor, and the presence of genes encoding virulence factor) of four LAB strains (Lactobacillus casei SJRP35, Leuconostoc citreum SJRP44, Lactobacillus delbrueckii subsp....
Authors: Rad AH1, Mehrabany EV, Alipoor B, Mehrabany LV.
Abstract: Probiotics are live bacteria which have frequently been reported to be beneficial in preventing a wide range of diseases as well as playing a major role in treating the existing ailments. Thus far, a variety of probiotic products have been developed which can be categorized into two groups: probiotic foods and supplements. Both foods and supplements have been able to confer the health benefits claimed for them. However, it is not known which one can be clinically more efficient, and to the best of our knowledge,...