Screening of Lactobacillus strains of domestic goose origin against bacterial poultry pathogens for use as probiotics.
Authors: Dec M1, Puchalski A2, Urban-Chmiel R2, Wernicki A2.
Abstract: Lactobacilli are natural inhabitants of human and animal mucous membranes, including the avian gastrointestinal tract. Recently, increasing attention has been given to their probiotic, health-promoting capacities, among which their antagonistic potential against pathogens plays a key role. A study was conducted to evaluate probiotic properties of Lactobacillus strains isolated from feces or cloacae of domestic geese. Among the 104 examined isolates, previously identified to the species level by whole-cell matrix-assisted...
Authors: Papastergiou V, Georgopoulos SD, Karatapanis S.
Abstract: Treatment of Helicobacter pylori (H. pylori) infection is paramount for the management of prevalent gastrointestinal disorders including peptic ulcer disease and gastric cancer. Due to the wide increase in prevalence of H. pylori resistance to antibiotics, clarithromycin-based triple therapies are not any more suitable for unconditional empiric use, and should not be recommended, unless local resistance to this antibiotic is low (< 20%). Alternative strategies have been proposed to overcome the issue of increasing...
General practitioners roles and experiences with functional foods containing probiotics and plant sterols.
Authors: Melling D.
Abstract: Aim The purpose of this research is to explore whether general practitioners have experiences with functional foods within their clinical practice.BACKGROUND: Previous research and editorials have suggested that general practitioners should have more involvement and knowledge of functional foods. This is due to the thought that functional foods may be consumed by their patients that could lead to other issues, such as patients not taking their medication. Therefore, research into general practitioners involvement...
Synbiotic Lactobacillus acidophilus NCFM and cellobiose does not affect human gut bacterial diversity but increases abundance of lactobacilli, bifidobacteria and branched-chain fatty acids: a randomized, double-blinded cross-over trial.
Authors: van Zanten GC1, Krych L, Röytiö H, Forssten S, Lahtinen SJ, Al-Soud WA, SÃ¸rensen S, Svensson B, Jespersen L, Jakobsen M.
Abstract: Probiotics, prebiotics and combinations thereof, i.e. synbiotics, have been reported to modulate gut microbiota of humans. In the present study effects of a novel synbiotic on the composition and metabolic activity of human gut microbiota were investigated. Healthy volunteers (n =18) were enrolled in a double-blinded, randomized, placebo-controlled crossover study and received synbiotic (Lactobacillus acidophilus NCFM (109 CFU) and cellobiose (5g)) or placebo daily for three weeks. Fecal samples were collected...
The effect of probiotics on functional constipation in adults: a systematic review and meta-analysis of randomized controlled trials.
Authors: Dimidi E, Christodoulides S, Fragkos KC, Scott SM, Whelan K.
Abstract: BACKGROUND: Functional constipation is a prevalent, burdensome gastrointestinal disorder whose treatment remains challenging. Probiotics have been increasingly investigated in its management.OBJECTIVE: The aim was to investigate the effect of probiotics on gut transit time, stool output, and constipation symptoms in adults with functional constipation via a systematic review and meta-analysis of randomized controlled trials (RCTs).DESIGN: Studies were identified by searching 4 electronic databases, back-searching...
Fourier transform infra-red spectroscopy and flow cytometric assessment of the antibacterial mechanism of action of aqueous extract of garlic (Allium sativum) against selected probiotic Bifidobacterium strains.
Authors: Booyens J, Thantsha MS.
Abstract: BACKGROUND: It is generally reported that garlic (Allium sativum) harms pathogenic but not beneficial bacteria. Although numerous studies supporting the alleged garlic effects on pathogens are available, there are limited studies to prove this claim for beneficial bacteria. We have recently shown that garlic exhibits antibacterial activity against probiotic bifidobacteria. The aim of the current study was to elucidate the mechanism of action of garlic clove extract (GCE) on Bifidobacterium bifidum LMG 11041,...
Effect on blood lipids of two daily servings of Camembert cheese. An intervention trial in mildly hypercholesterolemic subjects.
Authors: Schlienger JL1, Paillard F, Lecerf JM, Romon M, Bonhomme C, Schmitt B, Donazzolo Y, Defoort C, Mallmann C, Le Ruyet P, Bresson JL.
Abstract: As a concentrated source of saturated fat, cheese consumption is considered to be associated with increased cholesterolemia and generally forbidden in dietary guidelines for adults with hypercholesterolemia. The aim of this study was to evaluate the impact of saturated fatty acids on lipid parameters and blood pressure with regards to different types of dairy products: Camembert and full-fat yoghurt. One-hundred and fifty-nine moderate hypercholesterolemic subjects without treatment were instructed to consume...
[Nutrition and Metabolism Group of the Spanish Neonatology Society: Recommendations and evidence for dietary supplementation with probiotics in very low birth weight infants.]
Authors: Narbona López E1, Uberos Fernández J2, Armadá Maresca MI3, Couce Pico ML4, Rodríguez Martínez G5, Saenz de Pipaon M6.
Abstract: Clinical practice guidelines are an important tool for improving healthcare. In recent years there has been accumulating evidence on the impact of nutritional supplementation with probiotics in the very low birth weight infants. With no uniformity in microorganisms and strains used. The Spanish Neonatology Society (SENeo), through its Nutrition and Metabolism Group has undertaken to develop recommendations that will be useful as a guide for the neonatologist in this field.Copyright Â© 2014 Asociación EspaÃ±ola...
Characterization of lipoteichoic acid structures from three probiotic Bacillus strains: involvement of D-alanine in their biological activity.
Authors: Villéger R1, Saad N, Grenier K, Falourd X, Foucat L, Urdaci MC, Bressollier P, Ouk TS.
Abstract: Probiotics represent a potential strategy to influence the host's immune system thereby modulating immune response. Lipoteichoic Acid (LTA) is a major immune-stimulating component of Gram-positive cell envelopes. This amphiphilic polymer, anchored in the cytoplasmic membrane by means of its glycolipid component, typically consists of a poly (glycerol-phosphate) chain with D-alanine and/or glycosyl substitutions. LTA is known to stimulate macrophages in vitro, leading to secretion of inflammatory mediators such...
Melatonin Regulation as a Possible Mechanism for Probiotic (VSL#3) in Irritable Bowel Syndrome: A Randomized Double-Blinded Placebo Study.
Authors: Wong RK1, Yang C, Song GH, Wong J, Ho KY.
Abstract: BACKGROUND: Probiotics have treatment efficacy in irritable bowel syndrome (IBS), but the exact mechanism remains obscure. One hypothesis is the mediation of melatonin levels, leading to changes in IBS symptoms.AIM: The purpose of this study was to evaluate the effects of a probiotic, VSL#3, on symptoms, psychological and sleep parameters, and pain sensitivity in IBS, and relate these parameters to in vivo melatonin levels.METHODS: Forty-two IBS patients were randomly assigned to receive VSL#3 or placebo for...
Assessing the effects of exposure to environmental stress on some functional properties of Bifidobacterium animalis ssp. lactis.
Authors: Amund OD, Ouoba LI, Sutherland JP, Ghoddusi HB.
Abstract: This study assessed the effects of exposing a strain of Bifidobacterium animalis ssp. lactis to acid, bile and osmotic stresses on antagonistic properties, biofilm formation and antibiotic susceptibility/resistance profile. Exposure to each stress factor appeared to have no significant effect on the antagonism against Escherichia coli NCTC 12900 and Salmonella enterica serovar Enteritidis PT4. No suppression in biofilm formation due to exposure to stress was observed. Bile and osmotic stresses resulted in significantly...
The effect of yoghurt and its probiotics on blood pressure and serum lipid profile; a randomised controlled trial.
Authors: Ivey KL1, Hodgson JM2, Kerr DA3, Thompson PL4, Stojceski B5, Prince RL5.
Abstract: BACKGROUND AND AIMS: Despite strong mechanistic data, and promising results from inÂ vitro and animal studies, the ability of probiotic bacteria to improve blood pressure and serum lipid concentrations in humans remains uncertain. The aim of this study was to determine the effect of Lactobacillus acidophilus La5 and Bifidobacterium animalis subsp lactis Bb12, provided in either yoghurt or capsule form, on home blood pressure and serum lipid profile.METHODS AND RESULTS: Following a 3-week washout period, 156...
Authors: Nishiyama K1, Kawanabe A, Miyauchi H, Abe F, Tsubokawa D, Ishihara K, Yamamoto Y, Mukai T.
Abstract: The aim of this study was to assess the adhesion of Bifidobacterium strains to acidic carbohydrate moieties of porcine colonic mucin. Mucins were extracted and purified via gel filtration chromatography followed by density-gradient ultracentrifugation. The presence of sulfated and sialylated carbohydrates in mucins was shown by enzyme-linked immunosorbent assays using PGM34 and HMC31 monoclonal antibodies (mAbs), respectively. Adhesion of Bifidobacterium strains to mucin preparations was markedly affected by...
Authors: Li H1, Wang C2, Liu S2, Xu D2, Zhang J2, Chen H2.
Abstract: BACKGROUND: The causes and mechanisms of chronic diarrhea are complex. This study aimed to explore the relationship between chronic diarrhea with normal colonoscopy findings and terminal ileum lesions.METHODS: All cases were collected from January 2009 to June 2010. The 40 patients in the patient group had chronic diarrhea with normal colonoscopy findings. Those who had hyperthyroidism, diabetes, chronic pancreatitis, cirrhosis, atrophic gastritis, short bowel syndrome and connective tissue diseases had been...
Synbiotic yogurt-ice cream produced via incorporation of microencapsulated lactobacillus acidophilus (la-5) and fructooligosaccharide.
Authors: Ahmadi A1, Milani E2, Madadlou A3, Mortazavi SA4, Mokarram RR5, Salarbashi D1.
Abstract: Yogurt-ice cream is a nutritious product with a refreshing taste and durability profoundly longer than that of yogurt. The probiotic Lactobacillus acidophilus (La-5) cells either in free or encapsulated form were incorporated into yog-ice cream and their survivability were studied. Fructooligosaccharide (FOS) as a prebiotic compound at three levels (0, 4 & 8Â % w/w) was added to yogurt-ice cream mix and its effects on some chemical properties, overrun and firmness of product were evaluated. The higher...
Authors: Yang Y, Guo Y, Kan Q, Zhou XG, Zhou XY, Li Y.
Abstract: Necrotizing enterocolitis (NEC) is one of the most common acquired diseases of the gastrointestinal tract in preterm infants. Some randomized, controlled trials (RCTs) have indicated that probiotics may potentially lower the incidence of NEC and mortality. However, debate still remains about the safety of probiotics and their influence on normal infant growth. We performed this meta-analysis to assess the safety and benefits of probiotic supplementation in preterm infants. We searched in PubMed, Embase, and...
Effects of probiotic therapy on hepatic encephalopathy in patients with liver cirrhosis: an updated meta-analysis of six randomized controlled trials.
Authors: Xu J1, Ma R, Chen LF, Zhao LJ, Chen K, Zhang RB.
Abstract: BACKGROUND: Liver cirrhotic patients with hepatic encephalopathy have poor prognosis. Probiotics alter the intestinal microbiota and reduce the production of ammonia. We conducted a meta-analysis about the role of probiotics on liver cirrhotic patients with hepatic encephalopathy.DATA SOURCES: We collected the relevant literatures up to February 21, 2014 from databases of PubMed, EMBASE and the Cochrane Central Register of Controlled Trials. A statistical analysis was conducted by RevMan 5.2 and STATA 12.0 software.RESULTS:...
Authors: Mazzoli R1, Bosco F2, Mizrahi I3, Bayer EA4, Pessione E5.
Abstract: Lactic acid bacteria (LAB) have long been used in industrial applications mainly as starters for food fermentation or as biocontrol agents or as probiotics. However, LAB possess several characteristics that render them among the most promising candidates for use in future biorefineries in converting plant-derived biomass-either from dedicated crops or from municipal/industrial solid wastes-into biofuels and high value-added products. Lactic acid, their main fermentation product, is an attractive building block...
Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets.
Authors: Hu Y, Dun Y, Li S, Zhao S, Peng N, Liang Y.
Abstract: This research focused on the effects of different doses of Bacillus subtilis KN-42 on the growth performance, diarrhea incidence, faecal bacterial flora, and the relative number of Lactobacillus and Escherichia coli in faeces of weaned piglets to determine whether the strain can serve as a candidate antimicrobial growth promoter. A total of 360 piglets (initial body weight 7.14±0.63 kg) weaned at 26±2 days of age were randomly allotted to 5 treatment groups (4 pens per treatment with 18 pigs per pen) for a...
Authors: Dickinson B1, Surawicz CM.
Abstract: Diarrheal disease, which is most often caused by infectious pathogens, is a significant cause of morbidity and mortality worldwide, especially in children. This is particularly true in developing countries. Recent outbreaks of infectious diarrhea in developed countries, including the USA, are often attributed to food handling and distribution practices and highlight the need for continued vigilance in this area. Another common cause of infectious diarrhea, Clostridium difficile infection (CDI), has historically...
Authors: Batista D1, Raffals L.
Abstract: : Pouchitis is a common complication seen in patients with ulcerative colitis who undergo total proctocolectomy with ileal pouch anal anastomosis. Bacteria seem to play an important role in the development of pouchitis, although this role is not well defined. Because technology has advanced, we are able to apply molecular techniques to describe the structure and function of the pouch microbial community. In recent years, several studies have been performed comparing the pouch microbiota in patients with ulcerative...
Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice.
Authors: Sharma R1, Kapila R, Dass G, Kapila S.
Abstract: Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of comprehensive analysis of probiotic effects on aging physiology. The present study aimed at determining anti-immunosenescence potential of milk fermented with probiotic Lactobacillus rhamnosus (LR) in 16Â months old mice by concurrent analysis of immunosenescence...
Effect of probiotics on antioxidant and antimutagenic activities of crude peptide extract from yogurt.
Authors: Sah BN1, Vasiljevic T1, McKechnie S2, Donkor ON3.
Abstract: Search for bioactive peptides is intensifying because of the risks associated with the use of synthetic therapeutics, thus peptide liberation by lactic acid bacteria and probiotics has received a great focus. However, proteolytic capacity of these bacteria is strain specific. The study was conducted to establish proteolytic activity of Lactobacillus acidophilus (ATCC® 4356™), Lactobacillus casei (ATCC® 393™) and Lactobacillus paracasei subsp. paracasei (ATCC® BAA52™) in yogurt. Crude peptides were separated...
Isolation of Enterococcus faecium NM113, Enterococcus faceium NM213, and Lactobacillus casei NM512 as novel probiotics according to their immunomodulatory abilities.
Authors: Mansour NM1, Heine H, Abdou SM, Shenana ME, Zakaria MK, El-Diwany A.
Abstract: Probiotics, defined as living bacteria that are beneficial for human health, mainly function through their immunomodulatory abilities. Hence, these microorganisms have proven successful for treatment of diseases resulting from immune deregulation. In this study, we aimed to find novel candidates to improve and to complement current probiotic treatment strategies. Out of sixty lactic acid bacterial strains, which were isolated from faecal samples of healthy, full-term, breast-fed neonates, three were chosen because...
Imposition of encapsulated non-indigenous probiotics into intestine may disturb human core microbiome.
Authors: Barzegari A1, Eslami S2, Ghabeli E3, Omidi Y4.