Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain.
Authors: Nyanzi R1, Awouafack MD2, Steenkamp P3, Jooste PJ4, Eloff JN5.
Abstract: This study investigated the anti-Candida activity of methanol extracts from freeze-dried probiotic cells and the isolation of some constituents in the extracts. The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.25 and 5mg/ml after 48h of incubation. However, Lactococcus latics subsp. lactis strain X and Lactobacillus casei strain B extracts had an MIC of 10mg/ml after 48h of incubation. The extracts had fungistatic rather than fungicidal activity. These extracts...
Effect of alginate and chitosan on viability and release behavior of Bifidobacterium pseudocatenulatum G4 in simulated gastrointestinal fluid.
Authors: Kamalian N1, Mirhosseini H2, Mustafa S3, Manap MY2.
Abstract: The main aim of this study was to investigate the effect of different coating materials (i.e. Na-alginate and chitosan) on the viability and release behavior of Bifidobacterium pseudocatenulatum G4 in the simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). This study reports the viability of encapsulated B. pseudocatenulatum G4 coated using different alginate (2-4g/100mL) and chitosan (0.2-0.8g/100mL) concentrations. The results indicated that the highest concentration of alginate (4.4142g/100mL)...
Authors: Soukoulis C1, Behboudi-Jobbehdar S1, Yonekura L1, Parmenter C2, Fisk ID3.
Abstract: The concept of prebiotic edible films as effective vehicles for encapsulating probiotic living cells is presented. Four soluble fibres (inulin, polydextrose, glucose-oligosaccharides and wheat dextrin) were selected as prebiotic co-components of gelatine based matrices plasticised with glycerol and used for the immobilisation of Lactobacillusrhamnosus GG. The addition of prebiotics was associated with a more compact and uniform film structure, with no detectable interspaces or micropores; probiotic inclusion...
Improved probiotic viability in stress environments with post-culture of alginate-chitosan microencapsulated low density cells.
Authors: Song H1, Yu W2, Liu X3, Ma X4.
Abstract: In this study, probiotics (Saccharomyces cerevisiae Y235) were entrapped in alginate-chitosan microcapsules by emulsification/internal gelation technique. Two different encapsulation patterns were established as directly entrapped high density cells (dEHDC) and entrapped low density cells with culture (ELDCwc). The performance of microencapsulated cells, with free cells (FC) as control, was investigated against sequential stress environments of freeze-drying, storage, and simulated gastrointestinal fluids. After...
Authors: Batista D1, Raffals L.
Abstract: : Pouchitis is a common complication seen in patients with ulcerative colitis who undergo total proctocolectomy with ileal pouch anal anastomosis. Bacteria seem to play an important role in the development of pouchitis, although this role is not well defined. Because technology has advanced, we are able to apply molecular techniques to describe the structure and function of the pouch microbial community. In recent years, several studies have been performed comparing the pouch microbiota in patients with ulcerative...
Improvement in Th1/Th2 immune homeostasis, antioxidative status and resistance to pathogenic E. coli on consumption of probiotic Lactobacillus rhamnosus fermented milk in aging mice.
Authors: Sharma R1, Kapila R, Dass G, Kapila S.
Abstract: Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of comprehensive analysis of probiotic effects on aging physiology. The present study aimed at determining anti-immunosenescence potential of milk fermented with probiotic Lactobacillus rhamnosus (LR) in 16Â months old mice by concurrent analysis of immunosenescence...
Effect of probiotics on antioxidant and antimutagenic activities of crude peptide extract from yogurt.
Authors: Sah BN1, Vasiljevic T1, McKechnie S2, Donkor ON3.
Abstract: Search for bioactive peptides is intensifying because of the risks associated with the use of synthetic therapeutics, thus peptide liberation by lactic acid bacteria and probiotics has received a great focus. However, proteolytic capacity of these bacteria is strain specific. The study was conducted to establish proteolytic activity of Lactobacillus acidophilus (ATCC® 4356™), Lactobacillus casei (ATCC® 393™) and Lactobacillus paracasei subsp. paracasei (ATCC® BAA52™) in yogurt. Crude peptides were separated...
Effects of Bifidobacterial Supplementation to Pregnant Women and Infants in the Prevention of Allergy Development in Infants and on Fecal Microbiota.
Authors: Enomoto T1, Sowa M2, Nishimori K2, Shimazu S3, Yoshida A4, Yamada K5, Furukawa F6, Nakagawa T7, Yanagisawa N8, Iwabuchi N9, Odamaki T9, Abe F9, Nakayama J10, Xiao JZ9.
Abstract: Background: Probiotic administration may be a useful method for preventing allergies in infants; however, there have been controversial results about the efficacy. We investigated the effects of bifidobacterial supplementation on the risk of developing allergic diseases in the Japanese population. Methods: In an open trial, we gave Bifidobacterium breve M-16V and Bifidobacterium longum BB536 prenatally to 130 mothers beginning 1 month prior to delivery and postnatally to their infants for 6 months. Another 36...
A novel multi-strain probiotic and synbiotic supplement for prevention of Clostridium difficile infection in a murine model.
Authors: Kondepudi KK1, Ambalam P, Karagin PH, Nilsson I, Wadström T, Ljungh A.
Abstract: The protective effect of a multi-strain probiotic and synbiotic formulation was evaluated in C57BL/6 mice infected with Clostridium difficile (CD) NAP1/027. Antibiotic treated mice were divided into four groups. Group 1, fed with a synbiotic formulation consisting of Lactobacillus plantarum F44, L. paracasei F8, Bifidobacterium breve 46, B. lactis 8:8, galacto-oligosaccharides (GOS), isomalto-oligosaccharides (IMOS) and resistant starch (RS); group 2, fed with the same four probiotic strains as in group 1; group...
Orally delivered microencapsulated probiotic formulation favorably impacts polyp formation in APC (Min/+) model of intestinal carcinogenesis.
Authors: Urbanska AM1, Bhathena J, Cherif S, Prakash S.
Abstract: The development of intestinal polyps in an orthotopic colorectal mouse model, receiving a probiotic yogurt formulation containing microencapsulated live Lactobacillus acidophilus cells was investigated. The expression of various immunohistochemical markers namely CD8, Mac-1, Ki-67, and cleaved caspase-3, was evaluated. Results suggest that the probiotic formulation decreases overall intestinal inflammation. Mice receiving the probiotic formulation were found to develop almost two-fold fewer tumors in the small...
Isolation and molecular identification of lactic acid bacteria and Bifidobacterium spp. from faeces of the blue-fronted Amazon parrot in Brazil.
Authors: Allegretti L1, Revolledo L1, Astolfi-Ferreira CS1, Chacón JL1, Martins LM1, Seixas GH2, Ferreira AJ1.
Abstract: In Brazil, the blue-fronted Amazon parrot (Amazona aestiva) is a common pet. The faecal microbiota of these birds include a wide variety of bacterial species, the majority of which belong to the Gram-positive lactic acid bacteria (LAB) clade. The aim of this study was to investigate differences in the diversity and abundance of LAB and Bifidobacterium spp. in the cloacae between wild and captive birds and to select, identify and characterise LAB for consideration as a parrot probiotic. Cloacal swabs were collected...
Potential probiotic Bifidobacterium animalis ssp. lactis 420 prevents weight gain and glucose intolerance in diet-induced obese mice.
Authors: Stenman LK1, Waget A2, Garret C2, Klopp P2, Burcelin R2, Lahtinen S1.
Abstract: Alterations of the gut microbiota and mucosal barrier are linked with metabolic diseases. Our aim was to investigate the potential benefit of the potential probiotic Bifidobacterium animalis ssp. lactis 420 in reducing high-fat diet-induced body weight gain and diabetes in mice. In the obesity model, C57Bl/6J mice were fed a high-fat diet (60 energy %) for 12 weeks, and gavaged daily with B. lactis 420 (109 cfu) or vehicle. In the diabetes model, mice were fed a high-fat, ketogenic diet (72 energy % fat) for...
Effect of yogurt containing polydextrose, Lactobacillus acidophilus NCFM and Bifidobacterium lactis HN019: a randomized, double-blind, controlled study in chronic constipation.
Authors: Magro DO, de Oliveira LM, Bernasconi I, Ruela MD, Credidio L, Barcelos IK, Leal RF, Ayrizono MD, Fagundes JJ, Teixeira LD, Ouwehand AC, Coy CS.
Abstract: BACKGROUND: Constipation is a frequent complaint and the combination of a prebiotic and probiotics could have a potentially synergic effect on the intestinal transit. The present study therefore aims to investigate the combination of polydextrose (Litesse(R)), L. acidophilus NCFM(R) and B. lactis HN019 in a yogurt on intestinal transit in subjects who suffer from constipation.METHODS: Patients with constipation were randomly divided into two groups, Control Group (CG) and Treatment Group (TG), and had to eat...
Lactobacillus acidophilus Attenuates Down-regulation of DRA Function and Expression in Inflammatory Models.
Authors: Singh V1, Kumar A2, Raheja G, Anbazhagan AN3, Priyamvada S2, Saksena S2, Jhandier MN2, Gill RK2, Alrefai WA2, Borthakur A2, Dudeja PK4.
Abstract: Probiotics, including Lactobacilli, are commensal bacteria that have been used in clinical trials and experimental models for the prevention and treatment of diarrheal disorders. Our previous studies have shown that Lactobacillus acidophilus (LA) and its culture supernatant (CS) stimulated Cl-/HCO3 - exchange activity, acutely via an increase in the surface levels of DRA (Down-regulated in Adenoma, SLC26A3) and in long-term treatments via increasing its expression involving transcriptional mechanisms. However,...
Authors: Treven P1, TrmÄiÄ‡ A2, BogoviÄ MatijaÅ¡iÄ‡ B2, Rogelj I2.
Abstract: Lactobacillus gasseri K7 is an isolate from infant feces and has in vitro and in vivo established probiotic properties. Here, we report the improved version of the draft genome sequence, which comprises 8 scaffolds (13 contigs), a total length of 1.99Â Mb, and 1,841 predicted protein-coding sequences. Copyright Â© 2014 Treven et al....
Oral Administration of Bacillus subtilis Strain BSB3 Can Prevent Heat Stress-Related Adverse Effects in Rats.
Authors: Moore T1, Globa L, Pustovyy O, Vodyanoy V, Sorokulova I.
Abstract: AIMS: To determine the efficacy of Bacillus subtilis strain in prevention of heat stress related complications in rats.METHODS AND RESULTS: Male Sprague-Dawley rats weighing 250-300 g were treated by oral gavage with B. subtilis BSB3 strain or PBS twice a day for two days. Half of the rats of each group were exposed to heat stress (45o C, relative humidity 55% for 25 min) while the remaining rats were placed at identical conditions but at 25°C. Bacterial translocation, histological changes in the intestine,...
13th Annual Meeting of the Safety Pharmacology Society: focus on novel technologies and safety pharmacology frontiers.
Authors: Cavero I.
Abstract: Introduction: The 13th Annual Meeting of the Safety Pharmacology (SP) Society discussed novel therapeutic areas, recent regulatory developments, emerging technologies and non-pharmaceutical dairy products that may need SP evaluations for ensuring their human safety. Areas covered: The meeting honored Willem Einthoven, the father of electrocardiography. The Comprehensive in vitro Proarrhythmia Assay (CiPA) is an under-discussion proposal for replacing the International Conference on Hamonization (ICH) S7B guideline...
Antioxidant activity of exopolysaccharide from probiotic strain Enterococcus faecium (BDU7) from Ngari.
Authors: Abdhul K1, Ganesh M2, Shanmughapriya S2, Kanagavel M2, Anbarasu K3, Natarajaseenivasan K4.
Abstract: "Ngari" is a traditional fermented fish of Manipur and considered for its therapeutic value in healing stomach ulcers. In the present study, an attempt was made to isolate and identify an efficient antioxidant probiotic isolate from Ngari. BDU7 with potent antioxidant property was isolated and characterized. The isolate was identified by 16S rRNA genotyping as Enterococcus faecium. E. faecium showed auto aggregating and hydrophobicity of 72.7 and 54.8% respectively. The extrapolysaccharide (EPS) was extracted...
Authors: Gupta C, Prakash D.
Abstract: Nutrients present in various foods plays an important role in maintaining the normal functions of the human body. The major nutrients present in foods include carbohydrates, proteins, lipids, vitamins, and minerals. Besides these, there are some bioactive food components known as "phytonutrients" that play an important role in human health. They have tremendous impact on the health care system and may provide medical health benefits including the prevention and/or treatment of disease and various physiological...
Effect of Probiotics on Blood Pressure : A Systematic Review and Meta-Analysis of Randomized, Controlled Trials.
Authors: Khalesi S1, Sun J2, Buys N1, Jayasinghe R1.
Abstract: Previous human clinical trials have shown that probiotic consumption may improve blood pressure (BP) control. The aim of the present systematic review was to clarify the effects of probiotics on BP using a meta-analysis of randomized, controlled trials. PubMed, Scopus, Cochrane Library (Central), Physiotherapy Evidence Database, and Clinicaltrial.gov databases were searched until January 2014 to identify eligible articles. Meta-analysis using a random-effects model was chosen to analyze the impact of combined...
Authors: Kothari D1, Patel S, Goyal A.
Abstract: Functional oligosaccharides have emerged as valuable components of food and dietary supplements. Their resistance to digestion and fermentation by colonic microbes has given them the nutritional edge. Apart from implications as dietary fibers, sweeteners, and humectants, they are hailed as prebiotics. Their beneficial effects extend from antioxidant, anti-inflammatory, immunomodulatory, antiallergic, hypotensive, hyperlipemic, neuroprotective to anticancer. The rising popularity of bioactive oligosaccharides...
In vitro evaluation of the probiotic and functional potential of Lactobacillus strains isolated from fermented food and human intestine.
Authors: Ren D1, Li C2, Qin Y1, Yin R3, Du S4, Ye F4, Liu C4, Liu H4, Wang M4, Li Y4, Sun Y4, Li X4, Tian M4, Jin N5.
Abstract: This study aims to evaluate the functional and probiotic characteristics of eight indigenous Lactobacillus strains in vitro. The selected lactobacilli include strains of Lactobacillus casei subsp. casei, Lactobacillus salivarius subsp. salicinius, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus delbrueckii subsp. lactis, Lactobacillus delbrueckii subsp. bulgaricus, and Lactobacillus rhamnosus. All strains tolerated both pH 2 for 3 h and 1% bile salt for 24 h. The strains CICC 23174 and CGMCC...
Authors: Sarno M1, Lania G, Cuomo M, Nigro F, Passannanti F, Budelli A, Fasano F, Troncone R, Auricchio S, Barone MV, Nigro R, Nanayakkara M.
Abstract: Several recent reports describe a role of probiotics as a therapeutic approach for celiac disease (CD). Two undigested A-gliadin peptides, P31-43 and P57-68, are central to CD pathogenesis, inducing an innate and an adaptive immune response, respectively. They enter enterocytes and localize to vesicular compartment to induce their toxic/immunogenics effects. In this article, we tested the effect of probiotic Lactobacillus paracasei (LP) CBA L74 (International Depository Accession Number LMG P-24778), its supernatant...
A Comparative Pan-Genome Perspective of Niche-Adaptable Cell-Surface Protein Phenotypes in Lactobacillus rhamnosus.
Authors: Kant R1, Rintahaka J1, Yu X1, Sigvart-Mattila P2, Paulin L3, Mecklin JP4, Saarela M5, Palva A1, von Ossowski I1.
Abstract: Lactobacillus rhamnosus is a ubiquitously adaptable Gram-positive bacterium and as a typical commensal can be recovered from various microbe-accessible bodily orifices and cavities. Then again, other isolates are food-borne, with some of these having been long associated with naturally fermented cheeses and yogurts. Additionally, because of perceived health benefits to humans and animals, numerous L. rhamnosus strains have been selected for use as so-called probiotics and are often taken in the form of dietary...
Authors: Sadowska-Krawczenko I1, Paprzycka M2, Korbal P3, Wiatrzyk A4, Krysztopa-Grzybowska K4, Polak M4, Czajka U4, Lutyńska A4.
Abstract: A disseminated Lactobacillus rhamnosus GG ATCC 53103 infection was suspected in a 6 day-old newborn with intrauterine growth restriction (IUGR) symptoms, treated empirically with antibiotics and given L. rhamnosus GG with the aim of preventing antibiotic-associated gastrointestinal complications. The level of C-reactive protein on day 5 compared with day 2 was increased in spite of negative urine and cerebrospinal fluid cultures. The blood sampled on day 6 was found to be positive for lactobacilli, and the isolate...