The use of flow cytometry to accurately ascertain total and viable counts of Lactobacillus rhamnosus in chocolate.
Authors: Raymond Y1, Champagne CP2.
Abstract: The goals of this study were to evaluate the precision and accuracy of flow cytometry (FC) methodologies in the evaluation of populations of probiotic bacteria (Lactobacillus rhamnosus R0011) in two commercial dried forms, and ascertain the challenges in enumerating them in a chocolate matrix. FC analyses of total (FCT) and viable (FCV) counts in liquid or dried cultures were almost two times more precise (reproducible) than traditional direct microscopic counts (DCM) or colony forming units (CFU). With FC,...
Authors: Venema K.
Authors: Albin S1, Nowak-WÄ™grzyn A2.
Abstract: This article presents an overview of potential treatments of food allergy, with an emphasis on various forms of immunotherapy (including oral immunotherapy, sublingual immunotherapy, epicutaneous immunotherapy, immunotherapy with modified food antigens, and immunotherapy with a recombinant peanut vaccine). Allergen nonspecific treatments, such as Chinese herbal formulas, probiotics/prebiotics, helminths, monoclonal antibodies, and toll-like receptor agonists, are also summarized.Copyright Â© 2015 Elsevier...
Authors: Gomez Arango LF1, Barrett HL, Callaway LK, Nitert MD.
Abstract: Complications of pregnancy are associated with adverse outcomes for mother and baby in the short and long term. The gut microbiome has been identified as a key factor for maintaining health outside of pregnancy and could contribute to pregnancy complications. In addition, the vaginal and the recently revealed placental microbiome are altered in pregnancy and may play a role in pregnancy complications. Probiotic supplementation could help to regulate the unbalanced microflora composition observed in obesity and...
Lactobacillus rhamnosus CNCM I-3690 and the commensal bacterium Faecalibacterium prausnitzii A2-165 exhibit similar protective effects to induced barrier hyper-permeability in mice.
Authors: Laval L1, Martin R, Natividad JN, Chain F, Miquel S, Maredsous CD, Capronnier S, Sokol H, Verdu EF, Vlieg JE, BermÃºdez-Humarán LG, Smokvina T, Langella P.
Abstract: Impaired gut barrier function has been reported in a wide range of diseases and syndromes and in some functional gastrointestinal disorders. In addition, there is increasing evidence that suggests the gut microbiota tightly regulates gut barrier function and recent studies demonstrate that probiotic bacteria can enhance barrier integrity. Here, we aimed to investigate the effects of Lactobacillus rhamnosus CNCM I-3690 on intestinal barrier function. In vitro results using a Caco-2 monolayer cells stimulated...
The Protective Effects Of Selenium-enriched Probiotics On Carbon Tetrachloride-induced Liver Fibrosis In Rats.
Authors: Liu Y, Liu Q, Ye G, Khan A, Liu J, Gan F, Zhang X, Kumbhar S, Huang K.
Abstract: This study aimed to investigate the effects of Se-enriched probiotics (SP) on the liver ï¬brosis induced by CCl4 in rats. The results showed that SP signiï¬cantly decreased serum alanine aminotransferase (48.2%), aspartate aminotransferase (26.8%), hepatic hydroxyproline (29.3%) and malondialdehyde (30.1%) levels, but increased glutathione peroxidase (47.2%) and superoxide dismutase (39.4%) activites and glutathione levels (43.7%) (P < 0.05) in rats treated by CCl4. SP could alleviate hepatic inï¬‚ammation...
Authors: Munns KD1, Selinger LB, Stanford K, Guan L, Callaway TR, McAllister TA.
Abstract: Escherichia coli O157:H7 is a foodborne pathogen that causes illness in humans worldwide. Cattle are the primary reservoir of this bacterium, with the concentration and frequency of E. coli O157:H7 shedding varying greatly among individuals. The term "super-shedder" has been applied to cattle that shed concentrations of E. coli O157:H7â‰¥104 colony-forming units/g feces. Super-shedders have been reported to have a substantial impact on the prevalence and transmission of E. coli O157:H7 in the environment....
Authors: Raimondi S1, Anighoro A, Quartieri A, Amaretti A, Tomás-Barberán FA, Rastelli G, Rossi M.
Abstract: This study aimed to explore the capability of potentially probiotic bifidobacteria to hydrolyze chlorogenic acid into caffeic acid (CA), and to recognize the enzymes involved in this reaction. Bifidobacterium strains belonging to eight species occurring in the human gut were screened. The hydrolysis seemed peculiar of Bifidobacterium animalis, whereas the other species failed to release CA. Intracellular feruloyl esterase activity capable of hydrolyzing chlorogenic acid was detected only in B. animalis. In silico...
Role Of Probiotics And Functional Foods In Health: Gut Immune Stimulation By Two Probiotic Strains And A Potential Probiotic Yoghurt.
Authors: Galdeano CM, NuÃ±ez IN, Carmuega E, de LeBlanc AD, Perdigón G1.
Abstract: There are numerous reports that show the benefits on the health attributed to the probiotic consumptions. Most of the studies were performed using animal models and only some of them were validated in controlled human trials. The present review is divided in two sections. In the first section we describe how the probiotic microorganisms can interact with the intestinal epithelial cells that are the first line of cell in the mucosal site, focusing in the studies of two probiotic strains: Lactobacillus casei DN-114001(actually...
Authors: Nobili V1, Alkhouri N2, Alisi A1, Della Corte C1, Fitzpatrick E3, Raponi M1, Dhawan A3.
Abstract: Nonalcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome and is considered the most common form of chronic liver disease in children. Several factors contribute to NAFLD development, including race/ethnicity, genetic factors, environmental exposures, and alterations in the gut microbiome. The histologic spectrum of NAFLD ranges from simple steatosis to the more aggressive nonalcoholic steatohepatitis (NASH). Fibrosis and eventually cirrhosis can develop from NAFLD during...
Authors: Sarac F1, Salman T, Gun F, Celik A, Gurler N, Abbasoglu SD, Olgac V, Saygili A.
Abstract: OBJECTIVE: To investigate the effects of probiotics on bacterial translocation in the obstructive common bile duct with comparison to an enteral product containing arginine and glutamine.MATERIAL AND METHOD: In our study, 40 Sprague-Dawley rats each weighing 250-300Â g were used. Animals in Group 1 (sham) were laparatomized and fed standard chow supplemented with physiologic saline at daily doses of 2Â ml through orogastric tube for 7Â days. Common bile ducts of the animals in the other groups were ligated...
In vitro assessment of the gastrointestinal tolerance and immunomodulatory function of Bacillus methylotrophicus isolated from a traditional Korean fermented soybean food.
Authors: Sim I1, Koh JH, Kim DJ, Gu SH, Park A, Lim YH.
Abstract: AIMS: This study aimed to investigate the potential of Bacillus methylotrophicus as a probiotic.METHODS AND RESULTS: A Bacillus isolate designated strain C14 was isolated from Korean traditional fermented soybean paste (doenjang). The strain was identified and its physiological and biochemical properties were characterized. The gastrointestinal tolerance and immunomodulatory function of strain C14 were also investigated. Strain C14 was identified as Bacillus methylotrophicus by analysis of its biochemical properties...
Gas release-based prescreening combined with reversed-phase HPLC quantitation for efficient selection of high-Î³-aminobutyric acid (GABA)-producing lactic acid bacteria.
Authors: Wu Q1, Shah NP2.
Abstract: High Î³-aminobutyric acid (GABA)-producing lactobacilli are promising for the manufacture of GABA-rich foods and to synthesize GRAS (generally recognized as safe)-grade GABA. However, common chromatography-based screening is time-consuming and inefficient. In the present study, Korean kimchi was used as a model of lactic acid-based fermented foods, and a gas release-based prescreening of potential GABA producers was developed. The ability to produce GABA by potential GABA producers in de Man, Rogosa, and Sharpe...
Development of Lactobacillus paracasei harboring nucleic acid-hydrolyzing 3D8 scFv as a preventive probiotic against murine norovirus infection.
Authors: Hoang PM1, Cho S, Kim KE, Byun SJ, Lee TK, Lee S.
Abstract: The protein 3D8 single-chain variable fragment (3D8 scFv) has potential anti-viral activity due to its ability to penetrate into cells and hydrolyze nucleic acids. Probiotic Lactobacillus paracasei engineered to secrete 3D8 scFv for oral administration was used to test the anti-viral effects of 3D8 scFv against gastrointestinal virus infections. We found that injection of 3D8 scFv into the intestinal lumen resulted in the penetration of 3D8 scFv into the intestinal villi and lamina propria. 3D8 scFv secreted...
Gaseous emissions, growth performance and pork quality of pigs housed in deep-litter system compared to concrete-floor system.
Authors: Zhou C1, Hu J, Zhang B, Tan Z.
Abstract: This study measured gaseous emissions, growth performance and pork quality in a deep-litter system and concrete-floor system. Three hundred and twenty weaned piglets with an average body weight (BW) of 6.0â€‰±â€‰0.3â€‰kg were assigned randomly into three treatments. Treatments 1 and 2 included four pens with 20 pigs for each pen respectively in the deep-litter system, and the ratio of sawdust to chaff was 5:5 and 3:7 for treatments 1 and 2 respectively, the probiotics inoculated into the fermentation...
Authors: SÃ¸rensen SM1.
Abstract: INTRODUCTION: Acute infectious diarrhoea is common in travellers. The aim of this study was to examine the incidence of travellers' diarrhoea in a group of Danish doctors and to identify exposures that may confer protective benefits.METHODS: A total of 19 Danish infectious disease doctors, pediatricians and clinical microbiologists received a questionnaire regarding symptoms of infectious diarrhoea after a journey to Myanmar. The association between exposure and outcome was calculated as a relative risk.RESULTS:...
Authors: Kumar H1, Salminen S2, Verhagen H3, Rowland I4, Heimbach J5, BaÃ±ares S6, Young T7, Nomoto K8, Lalonde M9.
Abstract: Novel probiotics and prebiotics designed to manipulate the gut microbiota for improving health outcomes are in demand as the importance of the gut microbiota in human health is revealed. The regulations governing introduction of novel probiotics and prebiotics vary by geographical region. Novel foods and foods with health claims fall under specific regulations in several countries. The paper reviews the main requirements of the regulations in the EU, USA, Canada and Japan. We propose a number of areas that need...
Influence of Trace Elements Mixture on Bacterial Diversity and Fermentation Characteristics of Liquid Diet Fermented with Probiotics under Air-Tight Condition.
Authors: He Y1, Chen Z1, Liu X1, Wang C2, Lu W1.
Abstract: Cu2+, Zn2+, Fe2+ and I- are often supplemented to the diet of suckling and early weaning piglets, but little information is available regarding the effects of different Cu2+, Zn2+, Fe2+ and I- mixtures on bacteria growth, diversity and fermentation characteristics of fermented liquid diet for piglets. Pyrosequencing was performed to investigate the effect of Cu2+, Zn2+, Fe2+ and I- mixtures on the diversity, growth and fermentation characteristics of bacteria in the liquid diet fermented with Bacillus subtilis...
Significant Reduction in Helicobacter pylori Load in Humans with Non-viable Lactobacillus reuteri DSM17648: A Pilot Study.
Authors: Holz C1, Busjahn A, Mehling H, Arya S, Boettner M, Habibi H, Lang C.
Abstract: Reducing the amount of Helicobacter pylori in the stomach by selective bacterial-bacterial cell interaction was sought as an effective and novel method for combating the stomach pathogen. Lactobacillus reuteri DSM17648 was identified as a highly specific binding antagonist to H. pylori among more than 700 wild-type strains of Lactobacillus species. Applying a stringent screening procedure, the strain DSM17648 was identified as selective binder to H. pylori cells under in vivo gastric conditions. The strain DSM17648...
Authors: Bhat AR1, Irorere VU1, Bartlett T1, Hill D1, Kedia G1, Charalampopoulos D2, Nualkaekul S2, Radecka I3.
Abstract: A major hurdle in producing a useful probiotic food product is bacterial survival during storage and ingestion. The aim of this study was to test the effect of Î³-PGA immobilisation on the survival of probiotic bacteria when stored in acidic fruit juice. Fruit juices provide an alternative means of probiotic delivery, especially to lactose intolerant individuals. In addition, the survival of Î³-PGA-immobilised cells in simulated gastric juice was also assessed. Bifidobacteria strains (Bifidobacteria longum,...
Authors: Laleman I, Teughels W.
Abstract: During the last decade an increased interest in alternative, preventive, and therapeutic strategies in dentistry has arisen. Probiotics are living microorganisms which, if administered in sufficient amounts, provide a health benefit to the host. Their precise mechanisms of action have not been identified, but they are able to interfere with the imbalance occurring in biofilm-associated infections. In other fields of medicine, mainly in gastroenterology, their usefulness is already proven. Concerning oral threats,...
Effects of lactic acid bacteria on cardiac apoptosis are mediated by activation of the phosphatidylinositol-3 kinase/AKT survival-signalling pathway in rats fed a high-fat diet.
Authors: Wang HF1, Lin PP2, Chen CH1, Yeh YL3, Huang CC4, Huang CY5, Tsai CC6.
Abstract: Through a high-fat diet, obesity leads to cardiomyocyte dysfunction and apoptosis. In addition, there is no evidence that probiotics have potential health effects associated with cardiac apoptosis in obese rats. The present study aimed to explore the effects of probiotics on obesity and cardiac apoptosis in rats fed a high-fat diet (HF). Eightâ€‘weekâ€‘old male Wistar rats were separated randomly into five equally sized experimental groups: Normal diet (NC) and high-fat diet (HFC) groups, and high-fat...
Lactobacillus reuteri DSM 17938 for managing infant colic: protocol for an individual participant data meta-analysis.
Authors: Sung V1, Cabana MD2, D'Amico F3, Deshpande G4, Dupont C5, Indrio F6, Mentula S7, Partty A8, Savino F9, Szajewska H10, Tancredi D11.
Abstract: INTRODUCTION: Infant colic, or excessive crying of unknown cause in infants less than 3â€…months old, is common and burdensome. Its aetiology is undetermined, and consensus on its management is still lacking. Recent studies suggest a possible link between infant colic and gut microbiota, indicating probiotics to be a promising treatment. However, only a few strains have been tested, and results from randomised controlled trials are conflicting. It is important to clarify whether probiotics are effective...
Authors: Bertelli C1, Pillonel T1, Torregrossa A2, Prod'hom G3, Fischer CJ2, Greub G4, Giannoni E2.
Abstract: Administration of probiotics to premature newborns prevents necrotizing enterocolitis and reduces all cause mortality. In our hospital, we documented two cases of Bifidobacterium longum subspecies infantis bacteremia in newborns receiving probiotics. By comparative genomics, we confirmed that the strains isolated from each patient originated from the probiotics.Â© The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions,...
Lactobacillus crustorum KH: Novel Prospective Probiotic Strain Isolated from Iranian Traditional Dairy Products.
Authors: Sharafi H1, Derakhshan V, Paknejad M, Alidoust L, Tohidi A, Pornour M, Hajfarajollah H, Zahiri HS, Noghabi KA.
Abstract: In recent years, exploring novel probiotic strains for therapeutic intervention has been raised due to the significant increase in market demand. This study aimed to investigate the certain probiotic properties of 15 Lactobacillus isolates from Iranian traditional dairy products. Among them, a novel potential probiotic strain was isolated and identified as Lactobacillus crustorum. The characteristics of potential probiotics were examined in terms of resistance to acidity, bile, and salinity as well as antibiotic...